产品描述
This Human TNNI3 ELISA Kit was designed for the quantitative measurement of Human TNNI3 protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL .
别名
cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit; cardiomyopathy; dilated 2A (autosomal recessive) ELISA Kit; Cardiomyopathy; familial hypertrophic; 7; included ELISA Kit; CMD1FF ELISA Kit; CMD2A ELISA Kit; CMH7 ELISA Kit; cTnI ELISA Kit; Familial hypertrophic cardiomyopathy 7 ELISA Kit; MGC116817 ELISA Kit; RCM1 ELISA Kit; Tn1 ELISA Kit; Tni ELISA Kit; TNN I3 ELISA Kit; TNNC 1 ELISA Kit; TNNC1 ELISA Kit; TNNI3 ELISA Kit; TNNI3_HUMAN ELISA Kit; Troponin I ELISA Kit; Troponin I cardiac ELISA Kit; Troponin I cardiac muscle ELISA Kit; Troponin I cardiac muscle isoform ELISA Kit; Troponin I type 3 cardiac ELISA Kit; troponin I; cardiac 3 ELISA Kit; TroponinI ELISA Kit; Ttroponin I type 3 (cardiac) ELISA Kit
缩写
TNNI3
Uniprot No
P19429
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-100 | |
| 1:2 | Average % | 92 | |
| Range % | 83-101 | |
| 1:4 | Average % | 93 | |
| Range % | 85-103 | |
| 1:8 | Average % | 95 | |
| Range % | 90-102 | |
回收率
| The recovery of human cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 84-110 | |
| EDTA plasma (n=4) | 96 | 89-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.362 | 2.407 | 2.385 | 2.238 | | | 1500 | 1.421 | 1.453 | 1.437 | 1.290 | | | 750 | 0.852 | 0.846 | 0.849 | 0.702 | | | 375 | 0.489 | 0.512 | 0.501 | 0.354 | | | 187.5 | 0.323 | 0.336 | 0.330 | 0.183 | | | 94 | 0.280 | 0.286 | 0.283 | 0.136 | | | 47 | 0.228 | 0.227 | 0.228 | 0.081 | | | 0 | 0.146 | 0.147 | 0.147 | | |
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货期
3-5 working days