产品描述
This Rat PDGF-BB ELISA Kit was designed for the quantitative measurement of Rat PDGF-BB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39.063 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL.
缩写
PDGF-BB
灵敏度
9.77 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 104 | |
| Range % | 101-107 | |
| 1:2 | Average % | 97 | |
| Range % | 94-100 | |
| 1:4 | Average % | 88 | |
| Range % | 83-90 | |
| 1:8 | Average % | 89 | |
| Range % | 84-93 | |
回收率
| The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 105 | 101-109 | |
| EDTA plasma (n=4) | 110 | 107-113 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2500 | 2.794 | 2.753 | 2.774 | 2.578 | | | 1250 | 2.204 | 2.200 | 2.202 | 2.006 | | | 625 | 1.530 | 1.501 | 1.516 | 1.320 | | | 312.5 | 0.901 | 0.925 | 0.913 | 0.717 | | | 156.25 | 0.635 | 0.652 | 0.644 | 0.448 | | | 78.125 | 0.459 | 0.435 | 0.447 | 0.251 | | | 39.063 | 0.376 | 0.368 | 0.372 | 0.176 | | | 0 | 0.197 | 0.194 | 0.196 | | |
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货期
3-5 working days