产品描述
This Mouse PPARGC1A ELISA Kit was designed for the quantitative measurement of Mouse PPARGC1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
别名
Ppargc1a ELISA Kit; Pgc1 ELISA Kit; Pgc1a ELISA Kit; Ppargc1 ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC-1-alpha ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC-1-alpha ELISA Kit
缩写
PPARGC1A
Uniprot No
O70343
灵敏度
5.86 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPARGC1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 86-96 | |
| 1:2 | Average % | 104 | |
| Range % | 97-108 | |
| 1:4 | Average % | 94 | |
| Range % | 87-98 | |
| 1:8 | Average % | 99 | |
| Range % | 92-102 | |
回收率
| The recovery of mouse PPARGC1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 87-97 | |
| EDTA plasma (n=4) | 99 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.730 | 2.582 | 2.656 | 2.466 | | | 750 | 2.181 | 2.076 | 2.129 | 1.939 | | | 375 | 1.365 | 1.345 | 1.355 | 1.165 | | | 187.5 | 0.871 | 0.832 | 0.852 | 0.662 | | | 94 | 0.550 | 0.541 | 0.546 | 0.356 | | | 47 | 0.385 | 0.378 | 0.382 | 0.192 | | | 23.5 | 0.288 | 0.279 | 0.284 | 0.094 | | | 0 | 0.192 | 0.188 | 0.190 | | |
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货期
3-5 working days