产品描述
This Pig sP-Selectin ELISA Kit was designed for the quantitative measurement of Pig sP-Selectin protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
缩写
sP-Selectin
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 85-94 | |
| 1:2 | Average % | 95 | |
| Range % | 92-98 | |
| 1:4 | Average % | 87 | |
| Range % | 82-90 | |
| 1:8 | Average % | 93 | |
| Range % | 90-96 | |
回收率
| The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 83-90 | |
| EDTA plasma (n=4) | 85 | 82-89 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 800 | 1.828 | 1.968 | 1.898 | 1.750 | | | 400 | 1.179 | 1.236 | 1.208 | 1.060 | | | 200 | 0.734 | 0.757 | 0.746 | 0.598 | | | 100 | 0.485 | 0.476 | 0.481 | 0.333 | | | 50 | 0.311 | 0.323 | 0.317 | 0.169 | | | 25 | 0.251 | 0.257 | 0.254 | 0.106 | | | 12.5 | 0.188 | 0.182 | 0.185 | 0.037 | | | 0 | 0.145 | 0.151 | 0.148 | | |
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货期
3-5 working days