产品描述
This Human SCGB3A2 ELISA Kit was designed for the quantitative measurement of Human SCGB3A2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL .
别名
LU103 ELISA Kit; Pneumo secretory protein 1 ELISA Kit; PnSP-1 ELISA Kit; PNSP1 ELISA Kit; SCGB3A2 ELISA Kit; Secretoglobin family 3A member 2 ELISA Kit; SG3A2_HUMAN ELISA Kit; UGRP1 ELISA Kit; Uteroglobin-related protein 1 ELISA Kit
缩写
SCGB3A2
Uniprot No
Q96PL1
灵敏度
11.72 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 91-98 | |
| 1:2 | Average % | 88 | |
| Range % | 81-92 | |
| 1:4 | Average % | 104 | |
| Range % | 98-111 | |
| 1:8 | Average % | 97 | |
| Range % | 83-93 | |
回收率
| The recovery of human SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 85-99 | |
| EDTA plasma (n=4) | 95 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.703 | 2.752 | 2.728 | 2.606 | | | 1500 | 1.906 | 1.866 | 1.886 | 1.764 | | | 750 | 1.128 | 1.200 | 1.164 | 1.042 | | | 375 | 0.545 | 0.539 | 0.542 | 0.420 | | | 187.5 | 0.316 | 0.332 | 0.324 | 0.202 | | | 93.75 | 0.205 | 0.208 | 0.207 | 0.085 | | | 46.88 | 0.186 | 0.200 | 0.193 | 0.071 | | | 0 | 0.121 | 0.122 | 0.122 | | | | | | | | | | |
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货期
3-5 working days