产品描述
The mouse Lp-PLA2 Elisa kit is suitable for the quantitative measurement of mouse Lp-PLA2 in serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of mouse Lp-PLA2 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision, and recovery, as well as lot-to-lot consistency.
Lp-PLA2, encoded by the PLA2G7 gene, is a calcium-independent enzyme mainly secreted by macrophages and lymphocytes in atherosclerotic plaques. It is responsible for atherosclerotic plaque progression and instability by promoting inflammation. Lp-PLA2 breakdowns phospholipids in oxidized low-density lipoproteins (ox-LDL) into two proinflammatory and proatherogenic products, lysophosphatidylcholines (lysoPCs) and oxidized nonesterified fatty acids (oxNEFAs), which are involved in endothelial dysfunction and plaque inflammation that promote atherosclerosis. Increased Lp-PLA2 levels are strongly related to atherosclerosis-related diseases, including heart disease and ischemic stroke.
别名
Pla2g7 ELISA Kit; Pafah ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; EC 3.1.1.47 ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; PAF 2-acylhydrolase ELISA Kit
缩写
PLA2G7
Uniprot No
Q60963
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
87-102 |
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| 1:200 |
Average % |
95 |
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| Range % |
84-107 |
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| 1:400 |
Average % |
95 |
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| Range % |
83-102 |
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| 1:800 |
Average % |
96 |
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| Range % |
84-105 |
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回收率
| The recovery of mouse Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
86-105 |
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| EDTA plasma (n=4) |
96 |
88-109 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.715 |
2.652 |
2.684 |
2.565 |
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| 250 |
1.817 |
1.913 |
1.865 |
1.746 |
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| 125 |
1.113 |
1.165 |
1.139 |
1.020 |
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| 62.5 |
0.600 |
0.627 |
0.614 |
0.495 |
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| 31.2 |
0.380 |
0.393 |
0.387 |
0.268 |
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| 15.6 |
0.273 |
0.292 |
0.283 |
0.164 |
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| 7.8 |
0.192 |
0.202 |
0.197 |
0.078 |
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| 0 |
0.116 |
0.122 |
0.119 |
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货期
3-5 working days
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