产品描述

This Human TUBB3 ELISA Kit was designed for the quantitative measurement of Human TUBB3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

别名

beta 3 tubulin ELISA Kit; beta 4 ELISA Kit; beta-4 ELISA Kit; CDCBM ELISA Kit; CDCBM1 ELISA Kit; CFEOM3 ELISA Kit; CFEOM3A ELISA Kit; FEOM3 ELISA Kit; M(beta)3 ELISA Kit; M(beta)6 ELISA Kit; MC1R ELISA Kit; Neuron specific beta III Tubulin ELISA Kit; Neuron-specific class III beta-tubulin ELISA Kit; QccE-11995 ELISA Kit; QccE-15186 ELISA Kit; TBB3_HUMAN ELISA Kit; Tubb 3 ELISA Kit; TUBB3 ELISA Kit; TUBB4 ELISA Kit; Tubulin beta 3 ELISA Kit; Tubulin beta 3 chain ELISA Kit; Tubulin beta 4 ELISA Kit; Tubulin beta III ELISA Kit; Tubulin beta-3 chain ELISA Kit; Tubulin beta-4 chain ELISA Kit; Tubulin beta-III ELISA Kit; tuj 1 ELISA Kit; tuj1 ELISA Kit

缩写

TUBB3

Uniprot No

Q13509

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TUBB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		88-99
1:2		Average %		99
		Range %		93-105
1:4		Average %		86
		Range %		81-93
1:8		Average %		90
		Range %		84-98

回收率

The recovery of human TUBB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-97
EDTA plasma (n=4)		98		96-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.352 2.384 2.368 2.264 20 1.849 1.869 1.859 1.755 10 1.128 1.167 1.148 1.044 5 0.583 0.542 0.563 0.459 2.5 0.355 0.336 0.346 0.242 1.25 0.244 0.237 0.241 0.137 0.625 0.181 0.172 0.177 0.073 0 0.106 0.102 0.104

货期

3-5 working days