产品描述

This Human S100A10 ELISA Kit was designed for the quantitative measurement of Human S100A10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

42C ELISA Kit; AA409961 ELISA Kit; AL024248 ELISA Kit; Annexin II ligand ELISA Kit; Annexin II ligand; calpactin I; light polypeptide ELISA Kit; Annexin II tetramer (AIIt) p11 subunit ELISA Kit; Annexin II; light chain ELISA Kit; ANX2L ELISA Kit; ANX2LG ELISA Kit; Ca[1] ELISA Kit; CAL12 ELISA Kit; CAL1L ELISA Kit; Calpactin I light chain ELISA Kit; Calpactin I; p11 subunit ELISA Kit; Calpactin-1 light chain ELISA Kit; Cellular ligand of annexin II ELISA Kit; CLP11 ELISA Kit; GP11 ELISA Kit; MGC111133 ELISA Kit; Nerve growth factor-induced protein 42C ELISA Kit; OTTHUMP00000015269 ELISA Kit; OTTHUMP00000015270 ELISA Kit; p10 ELISA Kit; p10 protein ELISA Kit; p11 ELISA Kit; Protein S100 A10 ELISA Kit; Protein S100-A10 ELISA Kit; S100 calcium binding protein A10 (annexin II ligand; calpactin I; light polypeptide (p11)) ELISA Kit; S100 calcium binding protein A10 (calpactin) ELISA Kit; S100 calcium binding protein A10 ELISA Kit; S100 calcium-binding protein A10 ELISA Kit; S100a10 ELISA Kit; S10AA_HUMAN ELISA Kit

缩写

S100A10

Uniprot No

P60903

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		101
		Range %		97-104
1:2		Average %		108
		Range %		104-112
1:4		Average %		95
		Range %		91-98
1:8		Average %		89
		Range %		85-94

回收率

The recovery of human S100A10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		105		103-109
EDTA plasma (n=4)		96		93-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 2.044 2.125 2.085 1.936 10 1.582 1.621 1.602 1.453 5 1.146 1.154 1.150 1.001 2.5 0.679 0.731 0.705 0.556 1.25 0.466 0.451 0.459 0.310 0.625 0.352 0.362 0.357 0.208 0.312 0.235 0.222 0.229 0.080 0 0.145 0.152 0.149 　 .

货期

3-5 working days