产品描述
The Rat extracellular superoxide dismutase [Cu-Zn] (Cu/Zn-SOD/SOD3) ELISA kit is the ideal tool for researchers studying oxidative stress and metabolism in Rattus norvegicus. It is designed to detect Extracellular superoxide dismutase [Cu-Zn], encoded by the Sod3 gene, in a variety of sample types, including serum, plasma, cell culture supernates, and tissue homogenates. With a detection range of 15.6 pg/mL-1000 pg/mL and a sensitivity of 3.9 pg/mL, you can be confident in the accuracy of your results.
The assay principle is quantitative and employs a sandwich measurement technique. The assay time is 1-5h, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, providing reliable and precise readings.
This Rat extracellular superoxide dismutase [Cu-Zn] (Cu/Zn-SOD/SOD3) ELISA kit has been cited in over 10 research publications and is an excellent choice for researchers studying oxidative stress and metabolism.
别名
Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit
缩写
SOD3
Uniprot No
Q08420
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
93 |
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| Range % |
89-95 |
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| 1:400 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-97 |
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| 1:1600 |
Average % |
98 |
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| Range % |
91-101 |
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回收率
| The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
95 |
92-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.644 |
2.588 |
2.616 |
2.461 |
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| 500 |
2.264 |
2.344 |
2.304 |
2.149 |
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| 250 |
1.650 |
1.632 |
1.641 |
1.486 |
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| 125 |
1.044 |
1.028 |
1.036 |
0.881 |
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| 62.5 |
0.624 |
0.636 |
0.630 |
0.475 |
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| 31.2 |
0.383 |
0.368 |
0.376 |
0.221 |
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| 15.6 |
0.248 |
0.239 |
0.244 |
0.089 |
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| 0 |
0.156 |
0.154 |
0.155 |
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货期
3-5 working days
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