产品描述
The Rat Thioredoxin (Trx) ELISA Kit is a highly sensitive and specific assay for the quantitative detection of Trx in serum, plasma, and tissue homogenates from the Rat species.
Trx is a multifunctional protein that plays a vital role in maintaining cellular redox balance by acting as a reducing agent. Abnormal Trx levels have been linked to a variety of pathological conditions, including oxidative stresses，cancer, neurodegenerative diseases and cardiovascular disorders. This Trx ELISA Kit offers a reliable and efficient means of measuring Trx levels in biological samples, with a detection range of 3.12 ng/mL to 200 ng/mL and a sensitivity of 0.78 ng/mL.
The assay principle of our Trx ELISA Kit is based on the sandwich method. During the assay process, Trx in the sample is sandwiched between two Trx-specific antibodies, which are coated on the plate. The bound Trx is then detected using a detection antibody conjugated with HRP and a chromogenic substrate, producing a colorimetric signal that is directly proportional to the Trx concentration.
Our Trx ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in metabolism and oxidative stresses research,has been optimized for use with Rat samples. Its quantitative assay principle, coupled with its wide detection range and high sensitivity, make it an invaluable tool for researchers studying the physiological and pathological roles of Trx in cellular metabolism.
别名
Txn ELISA Kit; Txn1 ELISA Kit; Thioredoxin ELISA Kit; Trx ELISA Kit
缩写
TXN
Uniprot No
P11232
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Trx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 94-102 | |
| 1:2 | Average % | 91 | |
| Range % | 86-97 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 94 | |
| Range % | 90-100 | |
回收率
| The recovery of rat Trx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 102 | 97-109 | |
| EDTA plasma (n=4) | 96 | 91-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.412 | 2.312 | 2.362 | 2.242 | | | 100 | 1.722 | 1.632 | 1.677 | 1.557 | | | 50 | 1.168 | 1.193 | 1.181 | 1.061 | | | 25 | 0.726 | 0.749 | 0.738 | 0.618 | | | 12.5 | 0.480 | 0.451 | 0.466 | 0.346 | | | 6.25 | 0.286 | 0.275 | 0.281 | 0.161 | | | 3.12 | 0.229 | 0.214 | 0.222 | 0.102 | | | 0 | 0.122 | 0.118 | 0.120 | | |
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货期
3-5 working days
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