产品描述
This Mouse SFTPA1 ELISA Kit was designed for the quantitative measurement of Mouse SFTPA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
缩写
SFTPA1
Uniprot No
P35242
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1000 | Average % | 84 | |
| Range % | 80-92 | |
| 1:2000 | Average % | 96 | |
| Range % | 91-105 | |
| 1:4000 | Average % | 98 | |
| Range % | 93-103 | |
| 1:8000 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-95 | |
| EDTA plasma (n=4) | 93 | 90-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.637 | 2.714 | 2.676 | 2.549 | | | 100 | 2.331 | 2.368 | 2.350 | 2.223 | | | 50 | 1.847 | 1.901 | 1.874 | 1.747 | | | 25 | 1.273 | 1.267 | 1.270 | 1.143 | | | 12.5 | 0.845 | 0.869 | 0.857 | 0.730 | | | 6.25 | 0.542 | 0.583 | 0.563 | 0.436 | | | 3.12 | 0.322 | 0.317 | 0.320 | 0.193 | | | 0 | 0.128 | 0.126 | 0.127 | | |
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货期
3-5 working days