产品描述

The human P-Selectin (SELP) ELISA kit is suitable for the quantitative determination of human SELP in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

SELP is generated by activated platelets and endothelial cells and then stored in the α granules of platelets and in the Weibel-Palade bodies of endothelial cells (EC). It plays a critical role in the initial adhesion and rolling of platelets and leukocytes to sites of injury during inflammation via its receptor PSGL-1. SELP is also involved in hemostasis and thrombosis through PSGL-1 signaling in leukocytes and platelets and GPIbα in platelets. It is necessary for the progression of atherosclerosis.

别名

Antigen CD62 ELISA Kit; CD62 ELISA Kit; CD62 antigen-like family member P ELISA Kit; CD62P ELISA Kit; GMP 140 ELISA Kit; GMP-140 ELISA Kit; GMP140 ELISA Kit; GMRP ELISA Kit; Granule membrane protein 140 ELISA Kit; Granulocyte membrane protein ELISA Kit; GRMP ELISA Kit; LECAM 3 ELISA Kit; LECAM3 ELISA Kit; Leukocyte endothelial cell adhesion molecule 3 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LYAM3_HUMAN ELISA Kit; P Selectin ELISA Kit; P-selectin ELISA Kit; PADGEM ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; Platelet alpha granule membrane protein ELISA Kit; PSEL ELISA Kit; Selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit; SELP ELISA Kit

缩写

P-Selectin/CD62P

Uniprot No

P16109

灵敏度

0.225 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		86
		Range %		80-92
1:10		Average %		97
		Range %		91-105
1:20		Average %		98
		Range %		92-110
1:40		Average %		95
		Range %		86-98

回收率

The recovery of human P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		90-104
EDTA plasma (n=4)		96		87-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 60 2.305 2.368 2.337 2.269 30 1.795 1.805 1.800 1.732 15 1.146 1.187 1.167 1.099 7.5 0.653 0.689 0.671 0.603 3.75 0.344 0.358 0.351 0.283 1.8 0.245 0.264 0.255 0.187 0.9 0.110 0.116 0.113 0.045 0 0.066 0.069 0.068

货期

3-5 working days