产品描述
This Human TFPI2 ELISA Kit was designed for the quantitative measurement of Human TFPI2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
别名
Placental protein 5 ELISA Kit; PP5 ELISA Kit; REF1 ELISA Kit; Retinal pigment epithelium cell factor 1 ELISA Kit; TFPI 2 ELISA Kit; TFPI-2 ELISA Kit; TFPI2 ELISA Kit; TFPI2 PEN ELISA Kit; TFPI2_HUMAN ELISA Kit; Tissue factor pathway inhibitor 2 ELISA Kit
缩写
TFPI2
Uniprot No
P48307
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 93-104 | |
| 1:2 | Average % | 94 | |
| Range % | 90-99 | |
| 1:4 | Average % | 84 | |
| Range % | 80-89 | |
| 1:8 | Average % | 90 | |
| Range % | 85-95 | |
回收率
| The recovery of human TFPI-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-99 | |
| EDTA plasma (n=4) | 89 | 83-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.831 | 2.642 | 2.737 | 2.588 | | | 25 | 2.054 | 1.916 | 1.985 | 1.836 | | | 12.5 | 1.303 | 1.352 | 1.328 | 1.179 | | | 6.25 | 0.807 | 0.757 | 0.782 | 0.633 | | | 3.12 | 0.521 | 0.504 | 0.513 | 0.364 | | | 1.56 | 0.319 | 0.352 | 0.336 | 0.187 | | | 0.78 | 0.217 | 0.201 | 0.209 | 0.060 | | | 0 | 0.145 | 0.153 | 0.149 | | |
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货期
3-5 working days