产品描述

This human soluble cluster of differentiation 100 (sCD100) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human sCD100 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of sCD100 bound in the initial step.

CD100, also known as SEMA4D, plays a major role in the nervous and immune systems. SEMA4D has been shown to use two distinct receptors, plexin-B1 in the nervous system and CD72 in the immune system. SEMA4D is expressed at high levels in various tumor tissues, including head and neck squamous cell carcinoma, prostate cancer, and colon cancer. SEMA4D and its receptor Plexin-B1 are commonly dysregulated in cancers, suggesting roles in cancer progression, invasion, tumor angiogenesis, and skeletal metastasis.

别名

A8 ELISA Kit; BB 18 ELISA Kit; BB18 ELISA Kit; CD 100 ELISA Kit; CD100 ELISA Kit; CD100 antigen ELISA Kit; Coll 4 ELISA Kit; COLL4 ELISA Kit; Collapsin 4 ELISA Kit; Collapsin4 ELISA Kit; GR3 ELISA Kit; Leukocyte activation antigen CD100 ELISA Kit; M sema G ELISA Kit; MSEMA ELISA Kit; SEM4D_HUMAN ELISA Kit; Sema 4d ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ELISA Kit; Sema H ELISA Kit; SEMA J ELISA Kit; Sema4d ELISA Kit; Semacl 2 ELISA Kit; Semacl2 ELISA Kit; SemaH ELISA Kit; SEMAJ ELISA Kit; Semaphorin 4D ELISA Kit; Semaphorin C like 2 ELISA Kit; Semaphorin H ELISA Kit; Semaphorin J ELISA Kit; Semaphorin-4D ELISA Kit; Semaphorin4D ELISA Kit; SemaphorinJ ELISA Kit; Semcl 2 ELISA Kit; Semcl2 ELISA Kit

缩写

SEMA4D

Uniprot No

Q92854

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		89-102
1:2		Average %		93
		Range %		87-100
1:4		Average %		94
		Range %		88-102
1:8		Average %		92
		Range %		84-97

回收率

The recovery of human sCD100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		99		95-106
EDTA plasma (n=4)		92		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 1.850 1.835 1.843 1.755 4000 1.342 1.365 1.354 1.266 2000 0.834 0.900 0.867 0.779 1000 0.557 0.548 0.553 0.465 500 0.325 0.345 0.335 0.247 250 0.253 0.265 0.259 0.171 125 0.146 0.156 0.151 0.063 0 0.087 0.088 0.088

货期

3-5 working days