产品描述

This Human TNFAIP3 ELISA Kit was designed for the quantitative measurement of Human TNFAIP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL .

别名

A20 ELISA Kit; AISBL ELISA Kit; MGC104522 ELISA Kit; MGC138687 ELISA Kit; MGC138688 ELISA Kit; OTU domain containing protein 7C ELISA Kit; OTU domain-containing protein 7C ELISA Kit; OTUD7C ELISA Kit; Putative DNA binding protein A20 ELISA Kit; Putative DNA-binding protein A20 ELISA Kit; TNAP3_HUMAN ELISA Kit; TNF alpha-induced protein 3 ELISA Kit; TNFA1P2 ELISA Kit; TNFAIP 3 ELISA Kit; TNFAIP3 (A20) ELISA Kit; TNFAIP3 ELISA Kit; Tumor necrosis factor alpha induced protein 3 ELISA Kit; Tumor necrosis factor alpha-induced protein 3 ELISA Kit; Tumor necrosis factor induced protein 3 ELISA Kit; Tumor necrosis factor inducible protein A20 ELISA Kit; tumor necrosis factor, alpha-induced protein 3 ELISA Kit; Zinc finger protein A20 ELISA Kit

缩写

TNFAIP3

Uniprot No

P21580

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		86
		Range %		82-92
1:4		Average %		95
		Range %		89-100
1:8		Average %		97
		Range %		94-106

回收率

The recovery of human TNFAIP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		84-94
EDTA plasma (n=4)		99		95-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.496 2.458 2.477 2.344 750 1.658 1.788 1.723 1.590 375 1.085 1.195 1.140 1.007 187.5 0.690 0.685 0.688 0.555 94 0.454 0.467 0.461 0.328 47 0.320 0.321 0.321 0.188 23.5 0.231 0.245 0.238 0.105 0 0.135 0.131 0.133

货期

3-5 working days