产品描述
This Pig SERPINE1 ELISA Kit was designed for the quantitative measurement of Pig SERPINE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
SERPINE1 ELISA Kit; PAI1 ELISA Kit; PLANH1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写
SERPINE1
Uniprot No
P79335
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 83-93 | |
| 1:2 | Average % | 100 | |
| Range % | 95-104 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 95 | |
| Range % | 89-100 | |
回收率
| The recovery of pig PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 87-97 | |
| EDTA plasma (n=4) | 98 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.050 | 2.012 | 2.031 | 1.905 | | | 10 | 1.669 | 1.676 | 1.673 | 1.547 | | | 5 | 1.397 | 1.366 | 1.382 | 1.256 | | | 2.5 | 0.996 | 0.987 | 0.992 | 0.866 | | | 1.25 | 0.668 | 0.678 | 0.673 | 0.547 | | | 0.625 | 0.464 | 0.457 | 0.461 | 0.335 | | | 0.312 | 0.303 | 0.299 | 0.301 | 0.175 | | | 0 | 0.125 | 0.127 | 0.126 | | |
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货期
3-5 working days