产品描述
TGFB1是一种重要的细胞因子,广泛参与生物体的生长发育、免疫调节、组织修复和癌症等方面。TGFB1对于免疫细胞的增殖、分化、活化和功能调节都具有重要作用,也能够调节炎症反应和免疫耐受性。
华美生物所提供的Rat Transforming Growth Factor β1(TGF-β1) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的TGFB1,其灵敏度为1.56 pg/mL,检测范围为6.25 pg/mL-400 pg/mL。
别名
Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写
TGFB1
Uniprot No
P17246
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:200 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-101 |
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| 1:800 |
Average % |
102 |
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| Range % |
98-107 |
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回收率
| The recovery of rat TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-104 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.560 |
2.589 |
2.575 |
2.388 |
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| 200 |
2.262 |
2.299 |
2.281 |
2.094 |
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| 100 |
1.787 |
1.798 |
1.793 |
1.606 |
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| 50 |
1.098 |
1.076 |
1.087 |
0.900 |
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| 25 |
0.581 |
0.598 |
0.590 |
0.403 |
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| 12.5 |
0.421 |
0.452 |
0.437 |
0.250 |
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| 6.25 |
0.307 |
0.321 |
0.314 |
0.127 |
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| 0 |
0.186 |
0.188 |
0.187 |
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货期
3-5 working days