产品描述
TNF-α(Tumor Necrosis Factor-alpha,肿瘤坏死因子-alpha)是一种由多种免疫细胞产生的细胞因子,具有多种生物学活性,包括促炎和抗炎作用。TNF-α主要由巨噬细胞、淋巴细胞和树突细胞等细胞合成,并参与了炎症和免疫反应等生理过程。在炎症反应中,TNF-α能够引起局部炎症反应,增强炎症细胞的活性,并参与组织修复和免疫细胞的调节。然而,过度产生的TNF-α也与多种疾病的发生和发展相关,如类风湿关节炎、炎症性肠病和心血管疾病等。
华美生物所提供的Chicken Tumor necrosis factor α,TNF-α ELISA KIT属于ELISA检测试剂盒,采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的TNF-α,其灵敏度为0.27 pg/mL,检测范围为0.27 pg/mL-200 pg/mL。
别名
DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写
TNF-α
灵敏度
0.27 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
100 |
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| Range % |
95-105 |
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| 1:4 |
Average % |
87 |
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| Range % |
82-91 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
93-108 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.173 |
0.170 |
0.172 |
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| 66.7 |
0.259 |
0.251 |
0.255 |
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| 22.2 |
0.347 |
0.367 |
0.357 |
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| 7.41 |
0.452 |
0.488 |
0.470 |
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| 2.47 |
0.611 |
0.633 |
0.622 |
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| 0.82 |
0.809 |
0.855 |
0.832 |
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| 0.27 |
1.033 |
1.018 |
1.026 |
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| 0 |
1.748 |
1.707 |
1.728 |
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货期
3-5 working days