产品描述
The mouse prolactin (PRL) ELISA Kit is used to quantitatively measure PRL concentrations in mouse serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops inversely to the amount of PRL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
PRL is a sex hormone that is mainly synthesized and secreted by the lactotrophs of the pituitary and is responsible for the induction of lactation, the development of mammary alveoli, and milk production. It also exerts immunoregulatory properties. PRL signals by binding to PRL and activates a signaling cascade, including Jak/Stat, MAPK, Src, and PI3K/Akt pathways. PRL levels normally increase during pregnancy and breastfeeding and may rise due to physical stress, exercise, and certain medications and diseases. Higher-than-normal levels of PRL can cause certain symptoms, such as irregular periods, infertility, and erectile dysfunction.
别名
Prl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写
PRL
Uniprot No
P06879
灵敏度
0.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:2 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-102 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-102 |
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回收率
| The recovery of mouse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-100 |
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| EDTA plasma (n=4) |
96 |
89-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 25 |
0.165 |
0.174 |
0.170 |
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| 4.69 |
0.352 |
0.374 |
0.363 |
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| 0.78 |
0.573 |
0.589 |
0.581 |
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| 0.22 |
1.026 |
1.048 |
1.037 |
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| 0.06 |
1.562 |
1.574 |
1.568 |
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| 0 |
2.205 |
2.233 |
2.219 |
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货期
3-5 working days
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