产品描述
The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is the perfect solution for researchers studying the cardiovascular system. This quantitative assay kit allows for the detection of TF/F7 complex in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.
With a detection range of 15.6 pg/mL to 1000 pg/mL and a sensitivity of 3.9 pg/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.
The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of TF/F7 complex in rat samples. The assay principle is based on the sandwich method, providing high specificity and accuracy for the detection of this complex.
The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is ideal for researchers working in the cardiovascular field, and provides a powerful tool for the study of TF/F7 complex in rat samples.
缩写
TF
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 107 | | |
| Range % | 98-110 | | |
| 1:2 | Average % | 103 | | |
| Range % | 96-107 | | |
| 1:4 | Average % | 98 | | |
| Range % | 89-102 | | |
| 1:8 | Average % | 105 | | |
| Range % | 98-110 | | |
回收率
| The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 91 | 85-96 | | |
| EDTA plasma (n=4) | 102 | 95-107 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.708 | 2.673 | 2.691 | 2.589 | | | | 500 | 2.243 | 2.150 | 2.197 | 2.095 | | | | 250 | 1.419 | 1.469 | 1.444 | 1.342 | | | | 125 | 0.702 | 0.734 | 0.718 | 0.616 | | | | 62.5 | 0.412 | 0.425 | 0.419 | 0.317 | | | | 31.2 | 0.251 | 0.263 | 0.257 | 0.155 | | | | 15.6 | 0.167 | 0.172 | 0.170 | 0.068 | | | | 0 | 0.101 | 0.103 | 0.102 | | | |
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货期
3-5 working days
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