产品描述
The product CSB-E08433m is a sandwich ELISA kit developed to measure levels of mouse thrombin-antithrombin complex (TAT) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of TAT bound in the initial step.
TAT complex is a molecular complex composed of thrombin and antithrombin (AT), a primary thrombin inhibitor, in a 1:1 ratio. TAT is a sensitive parameter of the latent activator of the clotting pathway. Elevated TAT indicates excess thrombin production and serves as a marker reflecting prothrombotic status. And increased concentrations have been linked to vascular complications associated with diabetes.
别名
N/A
缩写
TAT
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:20 |
Average % |
95 |
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| Range % |
90-101 |
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| 1:40 |
Average % |
105 |
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| Range % |
96-112 |
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| 1:80 |
Average % |
93 |
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| Range % |
87-98 |
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回收率
| The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
93-100 |
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| EDTA plasma (n=4) |
90 |
85-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.658 |
2.456 |
2.557 |
2.374 |
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| 500 |
2.214 |
2.113 |
2.164 |
1.981 |
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| 250 |
1.503 |
1.522 |
1.513 |
1.330 |
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| 125 |
0.855 |
0.904 |
0.880 |
0.697 |
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| 62.5 |
0.542 |
0.563 |
0.553 |
0.370 |
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| 31.2 |
0.413 |
0.435 |
0.424 |
0.241 |
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| 15.6 |
0.291 |
0.302 |
0.297 |
0.114 |
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| 0 |
0.188 |
0.178 |
0.183 |
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货期
3-5 working days
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