产品描述

This Human RETNLB ELISA Kit was designed for the quantitative measurement of Human RETNLB protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.7 pg/mL.

别名

C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 ELISA Kit; CCGR ELISA Kit; Colon and small intestine specific cysteine rich protein ELISA Kit; Colon and small intestine-specific cysteine-rich protein ELISA Kit; Colon carcinoma related gene protein ELISA Kit; Colon carcinoma-related gene protein ELISA Kit; Cysteine rich secreted A12 alpha like protein 1 ELISA Kit; Cysteine rich secreted protein A12 alpha like 1 ELISA Kit; Cysteine rich secreted protein FIZZ2 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 1 ELISA Kit; Cysteine-rich secreted protein FIZZ2 ELISA Kit; FIZZ1 ELISA Kit; FIZZ2 ELISA Kit; Found in inflammatory zone 1 ELISA Kit; HXCP2 ELISA Kit; RELM beta ELISA Kit; RELMb ELISA Kit; RELMbeta ELISA Kit; Resistin like beta ELISA Kit; Resistin like protein beta ELISA Kit; Resistin-like beta ELISA Kit; RETNB_HUMAN ELISA Kit; RETNL2 ELISA Kit; Retnlb ELISA Kit; XCP2 ELISA Kit

缩写

RETNLB

Uniprot No

Q9BQ08

灵敏度

11.7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human RELM-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		96
		Range %		92-100
1:4		Average %		90
		Range %		85-94
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human RELM-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		95-106
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 3000 2.210 2.349 2.280 2.121 1500 1.647 1.713 1.680 1.521 750 1.129 1.092 1.111 0.952 375 0.701 0.674 0.688 0.529 187.5 0.438 0.457 0.448 0.289 94 0.352 0.369 0.361 0.202 47 0.224 0.235 0.230 0.071 0 0.156 0.162 0.159

货期

3-5 working days