产品描述

This Human ROCK2 ELISA Kit was designed for the quantitative measurement of Human ROCK2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 7.8 pg/mL.

别名

coiled-coil-containing protein kinase 2 ELISA Kit; KIAA0619 ELISA Kit; p164 ROCK 2 ELISA Kit; p164 ROCK-2 ELISA Kit; Rho associated coiled coil containing protein kinase 2 ELISA Kit; Rho associated protein kinase 2 ELISA Kit; Rho associated; coiled coil containing protein kinase II ELISA Kit; Rho kinase 2 ELISA Kit; Rho-associated ELISA Kit; Rho-associated protein kinase 2 ELISA Kit; ROCK 2 ELISA Kit; Rock II ELISA Kit; Rock2 ELISA Kit; ROCK2_HUMAN ELISA Kit; Rock2m ELISA Kit; ROK alpha ELISA Kit; ROKalpha ELISA Kit

缩写

ROCK2

Uniprot No

O75116

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Rock-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		85-103
1:2		Average %		94
		Range %		86-103
1:4		Average %		95
		Range %		86-102
1:8		Average %		88
		Range %		83-100

回收率

The recovery of human Rock-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		91-101
EDTA plasma (n=4)		96		86-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.352 2.417 2.385 2.268 1000 1.622 1.608 1.615 1.498 500 1.016 1.023 1.020 0.903 250 0.688 0.674 0.681 0.564 125 0.406 0.416 0.411 0.294 62.5 0.302 0.287 0.295 0.178 31.2 0.206 0.211 0.209 0.092 0 0.115 0.119 0.117

货期

3-5 working days