产品描述

This Human TNFSF9 ELISA Kit was designed for the quantitative measurement of Human TNFSF9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

别名

4 1BB L ELISA Kit; 4 1BB ligand ELISA Kit; 4 1BBL ELISA Kit; 4-1BB ligand ELISA Kit; 4-1BBL ELISA Kit; Cd137l ELISA Kit; Cd157l ELISA Kit; Homolog of mouse 4 1BB L ELISA Kit; Homolog of mouse 4 1BBL ELISA Kit; ILA ligand (TNF related) ELISA Kit; Ly63l ELISA Kit; Receptor 4 1BB ligand ELISA Kit; TNF superfamily member 9 ELISA Kit; TNFL9_HUMAN ELISA Kit; Tnfsf9 ELISA Kit; TNLG5A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 9 ELISA Kit; Tumor necrosis factor ligand 5A ELISA Kit; Tumor necrosis factor ligand superfamily member 9 ELISA Kit; Tumor necrosis factor superfamily member 9 ELISA Kit

缩写

TNFSF9

Uniprot No

P41273

灵敏度

4.7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFSF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		90-101
1:2		Average %		97
		Range %		92-105
1:4		Average %		95
		Range %		92-98
1:8		Average %		90
		Range %		87-96

回收率

The recovery of human TNFSF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		96-108
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1200 2.021 1.915 1.968 1.874   600 1.304 1.386 1.345 1.251   300 0.874 0.860 0.867 0.773   150 0.575 0.560 0.568 0.474   75 0.400 0.382 0.391 0.297   37.5 0.275 0.271 0.273 0.179   18.75 0.180 0.176 0.178 0.084   0 0.094 0.093 0.094

货期

3-5 working days