产品描述
The product CSB-E07000Rb is a sandwich ELISA kit developed to measure levels of rabbit transforming growth factor β1 (TGF-β1) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of TGF-β1 bound in the initial step.
TGF-β1 (TGFBI) is a pleiotropic cytokine ubiquitously expressed in most tissues with diverse biological functions including senescence, cell proliferation, apoptosis, tumor suppression, differentiation, migration, immunity, osteogenesis, adipogenesis, and wound healing. It importantly contributes to cardiac fibrosis by controlling differentiation, migration, and collagen secretion of cardiac myofibroblasts. TGF-β1 is the most abundant isoform in the mucosa of the gut and may play an important role in the postnatal adaptation of the gastrointestinal tract in suckling animals. It also regulates the intestinal development and function in post-weaning adaptation processes in the intestine of pigs.
别名
TGFBI ELISA Kit; Transforming growth factor-beta-induced protein ig-h3 ELISA Kit; Beta ig-h3 ELISA Kit; Kerato-epithelin ELISA Kit; RGD-containing collagen-associated protein ELISA Kit; RGD-CAP ELISA Kit
缩写
TGFBI
Uniprot No
Q95215
灵敏度
1.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
95 |
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| Range % |
87-107 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-105 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-100 |
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回收率
| The recovery of rabbit TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
91-102 |
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| EDTA plasma (n=4) |
95 |
89-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 2.86 |
0.056 |
0.058 |
0.057 |
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| 5.7 |
0.164 |
0.158 |
0.161 |
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| 11.4 |
0.382 |
0.371 |
0.377 |
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| 28.6 |
0.857 |
0.867 |
0.862 |
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| 50 |
1.639 |
1.621 |
1.630 |
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货期
3-5 working days
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