产品描述

This Human SERPINE2 ELISA Kit was designed for the quantitative measurement of Human SERPINE2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

GDN ELISA Kit; GDN_HUMAN ELISA Kit; GDNPF ELISA Kit; Glia derived nexin ELISA Kit; Glia-derived nexin ELISA Kit; Glial derived neurite promoting factor ELISA Kit; P17 ELISA Kit; Peptidase inhibitor 7 ELISA Kit; Pi-7 ELISA Kit; Plasminogen activator inhibitor type 1; member 2 ELISA Kit; PN-1 ELISA Kit; PN1 ELISA Kit; PNI ELISA Kit; Protease inhibitor 7 ELISA Kit; Protease nexin 1 ELISA Kit; Protease nexin I ELISA Kit; Serpin E2 ELISA Kit; Serpin family E member 2 ELISA Kit; Serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2 ELISA Kit; SERPINE 2 ELISA Kit; Serpine2 ELISA Kit

缩写

SERPINE2

Uniprot No

P07093

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		82-87
1:2		Average %		96
		Range %		93-99
1:4		Average %		98
		Range %		94-103
1:8		Average %		97
		Range %		93-100

回收率

The recovery of human SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		84-92
EDTA plasma (n=4)		107		103-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 2.281 2.108 2.195 2.065 10 1.405 1.432 1.419 1.289 5 0.822 0.801 0.812 0.682 2.5 0.483 0.468 0.476 0.346 1.25 0.365 0.372 0.369 0.239 0.625 0.287 0.298 0.293 0.163 0.312 0.222 0.220 0.221 0.091 0 0.131 0.129 0.130

货期

3-5 working days