产品描述
TNFRSF1B(肿瘤坏死因子受体超家族成员1B)是一种膜结合受体蛋白,其作用是与肿瘤坏死因子(TNF)结合并调节炎症反应、细胞凋亡和免疫应答。TNFRSF1B在多种疾病中发挥着重要的作用,包括炎症性疾病、自身免疫病、感染和肿瘤。在人体中,TNFRSF1B的表达广泛,尤其在免疫细胞和内皮细胞中表达较高。
华美生物所提供的Human soluble tumor necrosis factor receptor 2,sTNF-R2 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的TNFRSF1B,其灵敏度为7.8 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
CD120b ELISA Kit; p75 ELISA Kit; p75 TNF receptor ELISA Kit; p75TNFR ELISA Kit; p80 TNF alpha receptor ELISA Kit; p80 TNF-alpha receptor ELISA Kit; Soluble TNFR1B variant 1 ELISA Kit; TBP-2 ELISA Kit; TBPII ELISA Kit; TNF R II ELISA Kit; TNF R2 ELISA Kit; TNF R75 ELISA Kit; TNF-R2 ELISA Kit; TNF-RII ELISA Kit; TNFBR ELISA Kit; TNFR-II ELISA Kit; TNFR1B ELISA Kit; TNFR2 ELISA Kit; TNFR80 ELISA Kit; TNFRII ELISA Kit; Tnfrsf1b ELISA Kit; TNR1B_HUMAN ELISA Kit; Tumor necrosis factor beta receptor ELISA Kit; Tumor necrosis factor binding protein 2 ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; Tumor necrosis factor-binding protein 2 ELISA Kit
缩写
TNFRSF1B
Uniprot No
P20333
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
101 |
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| Range % |
95-105 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
97 |
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| Range % |
85-101 |
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| 1:8 |
Average % |
94 |
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| Range % |
85-99 |
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回收率
| The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
94-104 |
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| EDTA plasma (n=4) |
101 |
92-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.170 |
2.099 |
2.135 |
2.032 |
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| 1000 |
1.721 |
1.788 |
1.755 |
1.652 |
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| 500 |
1.272 |
1.243 |
1.258 |
1.155 |
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| 250 |
0.776 |
0.807 |
0.792 |
0.689 |
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| 125 |
0.487 |
0.501 |
0.494 |
0.391 |
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| 62.5 |
0.306 |
0.322 |
0.314 |
0.211 |
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| 31.25 |
0.209 |
0.213 |
0.211 |
0.108 |
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| 0 |
0.102 |
0.104 |
0.103 |
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货期
3-5 working days