产品描述

The human cyclophilin B (CyPB) ELISA Kit is used to quantitatively measure CyPB concentrations in human serum, plasma, cell lysates, and tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of CyPB in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

CyPB, also known as PPIB, is an immunophilin with cis-trans peptidyl-prolyl isomerase (PPIase) and chaperone-like activities. It functions to protect cells against aldosterone-induced oxidative stress, endoplasmic reticulum stress (ERS), and tubular cell injury via its PPIase activity. Furthermore, CyPB also participates in transcriptional regulation, protein secretion, immune response, apoptosis, and other cellular processes. Many studies have shown that CyPB plays an important role in the replication of several viruses, including the Japanese encephalitis virus (JEV), hepatitis C virus (HCV), and human papillomavirus type 16 (HPV 16).

别名

AA408962 ELISA Kit; AA553318 ELISA Kit; AI844835 ELISA Kit; Cphn 2 ELISA Kit; Cphn2 ELISA Kit; Cyclophilin B ELISA Kit; Cyclophilin like protein ELISA Kit; CyP 20b ELISA Kit; CYP S1 ELISA Kit; CYP-S1 ELISA Kit; CYPB ELISA Kit; EC 5.2.1.8 ELISA Kit; MGC14109 ELISA Kit; MGC2224 ELISA Kit; OI9 ELISA Kit; peptidyl prolyl cis trans isomerase B ELISA Kit; Peptidyl prolyl cis trans isomerase B precursor ELISA Kit; Peptidyl-prolyl cis-trans isomerase B ELISA Kit; peptidylprolyl isomerase B (cyclophilin B) ELISA Kit; Peptidylprolyl isomerase B ELISA Kit; PPIase ELISA Kit; PPIase B ELISA Kit; Ppib ELISA Kit; PPIB_HUMAN ELISA Kit; Rotamase ELISA Kit; Rotamase B ELISA Kit; S cyclophilin ELISA Kit; S-cyclophilin ELISA Kit; SCYLP ELISA Kit

缩写

PPIB

Uniprot No

P23284

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CyPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-92
1:2		Average %		90
		Range %		85-85
1:4		Average %		92
		Range %		88-98
1:8		Average %		98
		Range %		93-104

回收率

The recovery of human CyPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		95-109
EDTA plasma (n=4)		99		93-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.545 2.402 2.474 2.359 1000 1.732 1.846 1.789 1.674 500 1.187 1.164 1.176 1.061 250 0.604 0.639 0.622 0.507 125 0.392 0.374 0.383 0.268 62.5 0.268 0.280 0.274 0.159 31.25 0.201 0.208 0.205 0.090 0 0.118 0.112 0.115

货期

3-5 working days