产品描述
TNF (肿瘤坏死因子,Tumor necrosis factor) TNF是一种由单核细胞、巨噬细胞、T细胞、B细胞等多种细胞合成并分泌的细胞因子,在炎症反应和免疫调节中发挥着重要作用。TNF对炎症反应具有双重作用,既能够诱导和维持炎症反应,又能够抑制炎症反应。此外,TNF还参与细胞凋亡、细胞增殖、细胞分化等生物学过程。在某些疾病状态下,如风湿性关节炎、肝炎、肿瘤等,TNF的表达水平明显升高。
华美生物所提供的Rabbit Tumor necrosis factor α,TNFα ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测兔血清、血浆、组织匀浆、细胞裂解液样本中的TNF,其灵敏度为19.5 pg/mL,检测范围为78 pg/mL-5000 pg/mL。
别名
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写
TNF
Uniprot No
P04924
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-102 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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回收率
| The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
87-99 |
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| EDTA plasma (n=4) |
92 |
87-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.905 |
2.899 |
2.902 |
2.789 |
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| 2500 |
2.366 |
2.356 |
2.361 |
2.248 |
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| 1250 |
1.639 |
1.604 |
1.622 |
1.509 |
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| 625 |
0.971 |
0.939 |
0.955 |
0.842 |
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| 312 |
0.488 |
0.456 |
0.472 |
0.359 |
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| 156 |
0.275 |
0.259 |
0.267 |
0.154 |
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| 78 |
0.179 |
0.171 |
0.175 |
0.062 |
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| 0 |
0.116 |
0.110 |
0.113 |
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货期
3-5 working days