产品描述
The Rat Substance P, SP ELISA Kit is a perfect tool for neuroscience researchers studying Rattus norvegicus. This kit is specifically designed to detect Substance P in a variety of sample types, including serum, plasma, cell culture supernates, and tissue homogenates. With a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL, you can be sure of accurate and reliable results.
The assay principle is quantitative and employs a sandwich measurement technique. The assay time is 1-5h, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, providing precise and dependable readings.
This Rat Substance P, SP ELISA Kit has been cited in over 6 research publications and is an excellent choice for neuroscience researchers.
别名
N/A
缩写
SP
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 102 | |
| Range % | 98-106 | |
| 1:4 | Average % | 92 | |
| Range % | 87-95 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-97 | |
| EDTA plasma (n=4) | 96 | 91-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 1.738 | 1.682 | 1.710 | 1.618 | | | 200 | 1.376 | 1.444 | 1.410 | 1.318 | | | 100 | 1.066 | 1.045 | 1.056 | 0.964 | | | 50 | 0.672 | 0.666 | 0.669 | 0.577 | | | 25 | 0.448 | 0.434 | 0.441 | 0.349 | | | 12.5 | 0.302 | 0.296 | 0.299 | 0.207 | | | 6.25 | 0.152 | 0.157 | 0.155 | 0.063 | | | 0 | 0.093 | 0.091 | 0.092 | | |
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货期
3-5 working days
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