产品描述

This Mouse PIK3CA ELISA Kit was designed for the quantitative measurement of Mouse PIK3CA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

Pik3ca ELISA Kit; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit; PI3-kinase subunit alpha ELISA Kit; PI3K-alpha ELISA Kit; PI3Kalpha ELISA Kit; PtdIns-3-kinase subunit alpha ELISA Kit; EC 2.7.1.153 ELISA Kit; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit; PtdIns-3-kinase subunit p110-alpha ELISA Kit; p110alpha ELISA Kit; Phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit; Serine/threonine protein kinase PIK3CA ELISA Kit; EC 2.7.11.1 ELISA Kit

缩写

PIK3CA

Uniprot No

P42337

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		95-106
1:2		Average %		102
		Range %		96-107
1:4		Average %		87
		Range %		83-92
1:8		Average %		95
		Range %		89-99

回收率

The recovery of mouse PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-101
EDTA plasma (n=4)		102		93-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     1500 2.725 2.812 2.769 2.625     750 2.020 2.165 2.093 1.949     375 1.514 1.565 1.540 1.396     187.5 1.014 1.023 1.019 0.875     94 0.614 0.665 0.640 0.496     47 0.384 0.392 0.388 0.244     23.5 0.271 0.277 0.274 0.130     0 0.143 0.144 0.144

货期

3-5 working days