产品描述
This Mouse TACR1 ELISA Kit was designed for the quantitative measurement of Mouse TACR1 protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Tacr1; Tac1r; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1
缩写
TACR1
Uniprot No
P30548
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
93-106 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-99 |
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| 1:4 |
Average % |
94 |
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| Range % |
85-98 |
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| 1:8 |
Average % |
107 |
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| Range % |
96-110 |
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回收率
| The recovery of mouse SP-R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
81-95 |
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| EDTA plasma (n=4) |
101 |
92-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.226 |
2.187 |
2.207 |
2.042 |
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| 250 |
1.562 |
1.519 |
1.541 |
1.376 |
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| 125 |
1.006 |
1.044 |
1.025 |
0.860 |
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| 62.5 |
0.661 |
0.639 |
0.650 |
0.485 |
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| 31.2 |
0.489 |
0.523 |
0.506 |
0.341 |
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| 15.6 |
0.400 |
0.415 |
0.408 |
0.243 |
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| 7.8 |
0.274 |
0.270 |
0.272 |
0.107 |
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| 0 |
0.168 |
0.162 |
0.165 |
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货期
3-5 working days