产品描述

The Rat soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit is a powerful tool for quantitative analysis of the Receptor activator of nuclear factor-kB ligand (Tumor necrosis factor ligand superfamily member 11 protein) in rat samples.

This ELISA kit is specifically designed for the measurement of sRANKL protein levels in a variety of sample types, including serum, plasma, and cell culture supernates from Rattus norvegicus (Rat) species. With a detection range of 62.5 pg/mL to 4000 pg/mL and a sensitivity of 15.6 pg/mL, this ELISA kit can accurately detect even low levels of sRANKL protein.

The assay principle is based on a sandwich immunoassay, which involves the use of two specific antibodies that bind to different epitopes of the target protein. This approach ensures high specificity and sensitivity in the measurement of sRANKL levels. The assay can be completed in just 1-5 hours, with a sample volume of 50-100ul, making it a fast and convenient option for research. This ELISA kit has been cited in over 14 research articles, demonstrating its reliability and accuracy.

别名

Tnfsf11 ELISA Kit; Opgl ELISA Kit; Rankl ELISA Kit; Trance ELISA Kit; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; Osteoclast differentiation factor ELISA Kit; ODF ELISA Kit; Osteoprotegerin ligand ELISA Kit; OPGL ELISA Kit; Receptor activator of nuclear factor kappa-B ligand ELISA Kit; RANKL ELISA Kit; TNF-related activation-induced cytokine ELISA Kit; TRANCE ELISA Kit; CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11 ELISA Kit; membrane form; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; soluble form] ELISA Kit

缩写

TNFSF11

Uniprot No

Q9ESE2

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of rat sRANKL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		89-101
1:2		Average %		96
		Range %		91-105
1:4		Average %		97
		Range %		93-102
1:8		Average %		94
		Range %		90-99

回收率

The recovery of rat sRANKL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-100
EDTA plasma (n=4)		100		96-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 1.934 1.829 1.882 1.719 2000 1.194 1.139 1.167 1.004 1000 0.753 0.733 0.743 0.580 500 0.548 0.538 0.543 0.380 250 0.385 0.375 0.380 0.217 125 0.304 0.294 0.299 0.136 62.5 0.241 0.237 0.239 0.076 0 0.163 0.162 0.163

货期

3-5 working days