产品描述
This Human SPON2 ELISA Kit was designed for the quantitative measurement of Human SPON2 protein in serum, plasma, cerebrospinal fluid (CSF), cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; DIL 1 ELISA Kit; DIL-1 ELISA Kit; DIL1 ELISA Kit; DKFZp686G21139 ELISA Kit; FLJ16313 ELISA Kit; FLJ22401 ELISA Kit; FLJ34460 ELISA Kit; M spondin ELISA Kit; Mindin ELISA Kit; ORF Names: ELISA Kit; SPON 2 ELISA Kit; Spon2 ELISA Kit; SPON2_HUMAN ELISA Kit; Spondin 2 ELISA Kit; Spondin 2 extracellular matrix protein ELISA Kit; Spondin-2 ELISA Kit
缩写
SPON2
Uniprot No
Q9BUD6
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
1.825 |
1.897 |
1.861 |
1.744 |
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| 100 |
1.414 |
1.435 |
1.425 |
1.308 |
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| 50 |
0.996 |
0.981 |
0.989 |
0.872 |
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| 25 |
0.692 |
0.683 |
0.688 |
0.571 |
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| 12.5 |
0.423 |
0.458 |
0.441 |
0.324 |
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| 6.25 |
0.295 |
0.280 |
0.288 |
0.171 |
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| 3.12 |
0.206 |
0.216 |
0.211 |
0.094 |
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| 0 |
0.116 |
0.118 |
0.117 |
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货期
3-5 working days