产品描述

This Human SFRS1 ELISA Kit was designed for the quantitative measurement of Human SFRS1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

Alternative splicing factor 1 ELISA Kit; Alternative-splicing factor 1 ELISA Kit; arginine/serine-rich 1 ELISA Kit; ASF 1 ELISA Kit; ASF ELISA Kit; ASF-1 ELISA Kit; ASF1 ELISA Kit; FLJ53078 ELISA Kit; MGC5228 ELISA Kit; P33 subunit ELISA Kit; Pre mRNA splicing factor SF2 P33 subunit ELISA Kit; pre-mRNA-splicing factor SF2 ELISA Kit; Serine/arginine-rich splicing factor 1 ELISA Kit; SF2 ELISA Kit; SF2P33 ELISA Kit; SFRS1 ELISA Kit; Splicing factor 2 alternate splicing factor ELISA Kit; Splicing factor 2 ELISA Kit; Splicing factor ELISA Kit; Splicing factor arginine/serine rich 1 ELISA Kit; SR Splicing factor 1 ELISA Kit; SRp30a ELISA Kit; srsf1 ELISA Kit; SRSF1_HUMAN ELISA Kit

缩写

SFRS1

Uniprot No

Q07955

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SFRS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		88-97
1:2		Average %		96
		Range %		90-101
1:4		Average %		99
		Range %		96-104
1:8		Average %		102
		Range %		96-106

回收率

The recovery of human SFRS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		89-98
EDTA plasma (n=4)		98		95-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2000 1.875 1.969 1.922 1.766 1000 1.196 1.232 1.214 1.058 500 0.823 0.877 0.850 0.694 250 0.635 0.625 0.630 0.474 125 0.394 0.385 0.390 0.234 62.5 0.287 0.291 0.289 0.133 31.25 0.215 0.222 0.219 0.063 0 0.161 0.151 0.156

货期

3-5 working days