产品描述
This Human STX1A ELISA Kit was designed for the quantitative measurement of Human STX1A protein in serum, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
HPC 1 ELISA Kit; Neuron specific antigen HPC1 ELISA Kit; Neuron-specific antigen HPC-1 ELISA Kit; OTTHUMP00000174615 ELISA Kit; OTTHUMP00000174616 ELISA Kit; OTTHUMP00000174617 ELISA Kit; OTTHUMP00000174618 ELISA Kit; P35-1 ELISA Kit; STX1 ELISA Kit; STX1A ELISA Kit; STX1A_HUMAN ELISA Kit; SYN1A ELISA Kit; Syntaxin 1A (brain) ELISA Kit; Syntaxin 1A brain ELISA Kit; Syntaxin-1A ELISA Kit
缩写
STX1A
Uniprot No
Q16623
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human STX1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-102 | |
| 1:2 | Average % | 90 | |
| Range % | 85-96 | |
| 1:4 | Average % | 97 | |
| Range % | 91-105 | |
| 1:8 | Average % | 94 | |
| Range % | 86-100 | |
回收率
| The recovery of human STX1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-104 | |
| EDTA plasma (n=4) | 95 | 88-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.489 | 2.452 | 2.471 | 2.334 | | | 500 | 2.049 | 1.989 | 2.019 | 1.882 | | | 250 | 1.450 | 1.399 | 1.425 | 1.288 | | | 125 | 0.913 | 0.867 | 0.890 | 0.753 | | | 62.5 | 0.582 | 0.551 | 0.567 | 0.430 | | | 31.2 | 0.361 | 0.357 | 0.359 | 0.222 | | | 15.6 | 0.246 | 0.234 | 0.240 | 0.103 | | | 0 | 0.136 | 0.138 | 0.137 | | |
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货期
3-5 working days