产品描述

This Human sCD146 ELISA Kit was designed for the quantitative measurement of Human sCD146 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

别名

A32 antigen ELISA Kit; CD 146 ELISA Kit; CD146 ELISA Kit; CD146 antigen ELISA Kit; Cell surface glycoprotein MUC18 ELISA Kit; Cell surface glycoprotein P1H12 ELISA Kit; Gicerin ELISA Kit; Mcam ELISA Kit; Melanoma adhesion molecule ELISA Kit; Melanoma associated antigen A32 ELISA Kit; Melanoma associated antigen MUC18 ELISA Kit; Melanoma associated glycoprotein MUC18 ELISA Kit; Melanoma cell adhesion molecule ELISA Kit; Melanoma-associated antigen A32 ELISA Kit; Melanoma-associated antigen MUC18 ELISA Kit; MelCAM ELISA Kit; MUC 18 ELISA Kit; MUC18 ELISA Kit; MUC18_HUMAN ELISA Kit; S endo 1 ELISA Kit; S endo 1 endothelial associated antigen ELISA Kit; S-endo 1 endothelial-associated antigen ELISA Kit

缩写

sCD146

Uniprot No

P43121

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD146 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		85
		Range %		81-90
1:10		Average %		96
		Range %		93-99
1:20		Average %		87
		Range %		83-90
1:40		Average %		104
		Range %		101-104

回收率

The recovery of human sCD146 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		93-99
EDTA plasma (n=4)		98		95-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.952 2.968 2.960 2.777 40 2.383 2.375 2.379 2.196 20 1.615 1.646 1.631 1.448 10 0.972 0.999 0.986 0.803 5 0.595 0.578 0.587 0.404 2.5 0.437 0.468 0.453 0.270 1.25 0.364 0.357 0.361 0.178 0 0.182 0.184 0.183

货期

3-5 working days