产品描述

This Human PSMD4 ELISA Kit was designed for the quantitative measurement of Human PSMD4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

别名

26S protease subunit S5a ELISA Kit; 26S proteasome non ATPase regulatory subunit 4 ELISA Kit; 26S proteasome non-ATPase regulatory subunit 4 ELISA Kit; 26S proteasome regulatory subunit rpn10 ELISA Kit; 26S proteasome regulatory subunit S5A ELISA Kit; AF 1 ELISA Kit; AF ELISA Kit; AF1 ELISA Kit; Angiocidin ELISA Kit; Antisecretory factor 1 ELISA Kit; ASF ELISA Kit; DS5a ELISA Kit; MCB 1 ELISA Kit; MCB1 ELISA Kit; Multiubiquitin chain binding protein ELISA Kit; Multiubiquitin chain-binding protein ELISA Kit; OTTHUMP00000059963 ELISA Kit; Prosome macropain ELISA Kit; Proteasome (prosome macropain) 26S subunit non ATPase 4 ELISA Kit; Proteasome 19S S5A ELISA Kit; Proteasome 26S non ATPase subunit 4 ELISA Kit; Proteasome 26S subunit non ATPase 4 ELISA Kit; PSMD 4 ELISA Kit; Psmd4 ELISA Kit; PSMD4_HUMAN ELISA Kit; pUB R5 ELISA Kit; pUBR5 ELISA Kit; Rpn 10 ELISA Kit; Rpn10 ELISA Kit; RPN10 homolog ELISA Kit; S5A ELISA Kit; S5a/antisecretory factor protein ELISA Kit

缩写

PSMD4

Uniprot No

P55036

灵敏度

8.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PSMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		84-94
1:2		Average %		102
		Range %		97-107
1:4		Average %		98
		Range %		88-102
1:8		Average %		99
		Range %		92-104

回收率

The recovery of human PSMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		91-102
EDTA plasma (n=4)		95		88-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     2200 2.302 2.234 2.268 2.120     1100 1.560 1.488 1.524 1.376     550 0.901 0.932 0.917 0.769     275 0.502 0.511 0.507 0.359     137.5 0.293 0.287 0.290 0.142     68.8 0.249 0.246 0.248 0.100     34.4 0.196 0.190 0.193 0.045     0 0.152 0.144 0.148

货期

3-5 working days