产品描述
PGE2(Prostaglandin E2)是一种重要的生理调节因子,它可以影响许多生物过程,包括炎症反应、免疫调节、血管扩张和疼痛传递等。PGE2是一种强烈的炎症介质,其含量水平在许多疾病过程中受到调节,如炎症性肠病、类风湿性关节炎、心血管疾病等。
华美生物所提供的Rat Prostaglandin E2,PG-E2 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的PG-E2,其灵敏度为0.25 pg/mL,检测范围为0.5 pg/mL-100 pg/mL。
缩写
PG-E2
灵敏度
0.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
91-99 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:4 |
Average % |
93 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
105 |
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| Range % |
99-109 |
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回收率
| The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-99 |
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| EDTA plasma (n=4) |
101 |
96-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 100 |
0.228 |
0.224 |
0.226 |
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| 25 |
0.365 |
0.348 |
0.357 |
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| 8 |
0.560 |
0.539 |
0.550 |
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| 2 |
0.813 |
0.787 |
0.800 |
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| 0.5 |
1.258 |
1.234 |
1.246 |
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| 0 |
2.031 |
2.176 |
2.104 |
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货期
3-5 working days