产品描述
小鼠S100-A4蛋白(S100A4) ELISA kit用于定量检测血清、血浆、细胞培养上清、组织匀浆中的小鼠 S100A4 蛋白。该ELISA检测试剂盒采用双抗夹心法,其检测范围为62.5 pg/mL-4000 pg/mL,灵敏度为15.6 pg/mL。
别名
S100a4 ELISA Kit; Capl ELISA Kit; Mts1 ELISA Kit; Protein S100-A4 ELISA Kit; Metastasin ELISA Kit; Metastatic cell protein ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein 18A2 ELISA Kit; Protein Mts1 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit
缩写
S100A4
Uniprot No
P07091
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:200 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:400 |
Average % |
103 |
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| Range % |
99-108 |
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| 1:800 |
Average % |
98 |
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| Range % |
94-105 |
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回收率
| The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-96 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.418 |
2.524 |
2.471 |
2.277 |
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| 2000 |
1.854 |
1.917 |
1.886 |
1.692 |
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| 1000 |
1.360 |
1.294 |
1.327 |
1.133 |
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| 500 |
0.912 |
0.893 |
0.903 |
0.709 |
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| 250 |
0.659 |
0.627 |
0.643 |
0.449 |
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| 125 |
0.401 |
0.421 |
0.411 |
0.217 |
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| 62.5 |
0.312 |
0.297 |
0.305 |
0.111 |
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| 0 |
0.197 |
0.190 |
0.194 |
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货期
3-5 working days