产品描述
This Mouse SDF1A/CXCL12 ELISA Kit was designed for the quantitative measurement of Mouse SDF1A/CXCL12 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Cxcl12 ELISA Kit; Sdf1 ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; SDF-1 ELISA Kit; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; TPAR1 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; PBSF ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; TLSF ELISA Kit
缩写
SDF-1α/SDF1A
Uniprot No
P40224
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 101 | |
| Range % | 99-105 | |
| 1:2 | Average % | 96 | |
| Range % | 91-98 | |
| 1:4 | Average % | 89 | |
| Range % | 84-94 | |
| 1:8 | Average % | 98 | |
| Range % | 95-104 | |
回收率
| The recovery of mouse SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 95-101 | |
| EDTA plasma (n=4) | 92 | 87-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.358 | 2.467 | 2.413 | 2.258 | | | 5 | 2.002 | 1.977 | 1.990 | 1.835 | | | 2.5 | 1.355 | 1.425 | 1.390 | 1.235 | | | 1.25 | 0.815 | 0.858 | 0.837 | 0.682 | | | 0.625 | 0.510 | 0.525 | 0.518 | 0.363 | | | 0.312 | 0.345 | 0.373 | 0.359 | 0.204 | | | 0.156 | 0.271 | 0.280 | 0.276 | 0.121 | | | 0 | 0.156 | 0.153 | 0.155 | | |
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货期
3-5 working days