产品描述

This Human TXNRD1 ELISA Kit was designed for the quantitative measurement of Human TXNRD1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

cytoplasmic ELISA Kit; Gene associated with retinoic and IFN-induced mortality 12 protein ELISA Kit; Gene associated with retinoic and interferon-induced mortality 12 protein ELISA Kit; Gene associated with retinoid IFN induced mortality 12 protein ELISA Kit; GRIM 12 ELISA Kit; GRIM-12 ELISA Kit; GRIM12 ELISA Kit; KDRF ELISA Kit; KM 102 derived reductase like factor ELISA Kit; KM-102-derived reductase-like factor ELISA Kit; MGC9145 ELISA Kit; Oxidoreductase ELISA Kit; Thioredoxin reductase 1 ELISA Kit; Thioredoxin reductase 1 cytoplasmic ELISA Kit; Thioredoxin reductase GRIM 12 ELISA Kit; Thioredoxin reductase TR1 ELISA Kit; TR 1 ELISA Kit; TR ELISA Kit; TR1 ELISA Kit; TRXR 1 ELISA Kit; TRXR1 ELISA Kit; TRXR1_HUMAN ELISA Kit; TXNR ELISA Kit; TXNRD 1 ELISA Kit; Txnrd1 ELISA Kit

缩写

TXNRD1

Uniprot No

Q16881

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TrxR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		99
		Range %		87-104
1:2		Average %		101
		Range %		94-105
1:4		Average %		94
		Range %		85-99
1:8		Average %		100
		Range %		84-105

回收率

The recovery of human TrxR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		83-94
EDTA plasma (n=4)		102		91-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 1.994 1.943 1.969 1.787 10 1.555 1.501 1.528 1.346 5 1.091 1.062 1.077 0.895 2.5 0.776 0.784 0.780 0.598 1.25 0.484 0.476 0.480 0.298 0.625 0.359 0.347 0.353 0.171 0.312 0.270 0.263 0.267 0.085 0 0.185 0.179 0.182

货期

3-5 working days