产品描述
This Mouse TGFA ELISA Kit was designed for the quantitative measurement of Mouse TGFA protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
Tgfa ELISA Kit; Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha ELISA Kit; TGF-alpha ELISA Kit; EGF-like TGF ELISA Kit; ETGF ELISA Kit; TGF type 1)] ELISA Kit
缩写
TGFA
Uniprot No
P48030
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 91-103 | |
| 1:2 | Average % | 99 | |
| Range % | 93-103 | |
| 1:4 | Average % | 100 | |
| Range % | 96-105 | |
| 1:8 | Average % | 95 | |
| Range % | 88-98 | |
回收率
| The recovery of mouse TGF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-96 | |
| EDTA plasma (n=4) | 92 | 87-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.891 | 2.874 | 2.883 | 2.720 | | | 400 | 2.451 | 2.332 | 2.392 | 2.229 | | | 200 | 1.991 | 1.887 | 1.939 | 1.776 | | | 100 | 1.352 | 1.345 | 1.349 | 1.186 | | | 50 | 0.707 | 0.692 | 0.700 | 0.537 | | | 25 | 0.448 | 0.431 | 0.440 | 0.277 | | | 12.5 | 0.288 | 0.268 | 0.278 | 0.115 | | | 0 | 0.165 | 0.161 | 0.163 | | |
|
货期
3-5 working days