产品描述

This Human TREML1 ELISA Kit was designed for the quantitative measurement of Human TREML1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

dJ238O23.3 ELISA Kit; GLTL1825 ELISA Kit; MGC119173 ELISA Kit; PRO3438 ELISA Kit; TLT 1 ELISA Kit; TLT-1 ELISA Kit; TLT1 ELISA Kit; TREM like transcript 1 ELISA Kit; Trem like transcript 1 protein ELISA Kit; Trem-like transcript 1 protein ELISA Kit; TREML 1 ELISA Kit; Treml1 ELISA Kit; Triggering receptor expressed on myeloid cells like 1 ELISA Kit; Triggering receptor expressed on myeloid cells like protein 1 ELISA Kit; Triggering receptor expressed on myeloid cells-like protein 1 ELISA Kit; TRML1_HUMAN ELISA Kit

缩写

TREML1

Uniprot No

Q86YW5

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TREML1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-94
1:2		Average %		100
		Range %		95-104
1:4		Average %		97
		Range %		86-102
1:8		Average %		102
		Range %		94-107

回收率

The recovery of human TREML1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		84-97
EDTA plasma (n=4)		96		87-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     4000 2.396 2.304 2.350 2.196     2000 2.017 1.976 1.997 1.843     1000 1.536 1.500 1.518 1.364     500 1.006 0.995 1.001 0.847     250 0.569 0.551 0.560 0.406     125 0.355 0.349 0.352 0.198     62.5 0.275 0.266 0.271 0.117     0 0.151 0.157 0.154

货期

3-5 working days