产品描述
Plg(Plasminogen)是一种由肝脏合成并广泛存在于血液中的单链糖蛋白,是体内溶酶原的前体,可被活化成为溶解纤维蛋白和其他基质蛋白,参与体内纤维蛋白和其他蛋白质的降解和清除。Plg水平的升高与炎症、血栓形成、肿瘤和其他疾病的发生相关。
华美生物所提供的Rat Plasminogen (Plg) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的Plg,其灵敏度为15.6 ng/mL,检测范围为62.5 ng/mL-4000 ng/mL。
别名
Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
缩写
Plg
Uniprot No
Q01177
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
86-103 |
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| 1:200 |
Average % |
91 |
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| Range % |
83-99 |
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| 1:400 |
Average % |
95 |
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| Range % |
82-97 |
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| 1:800 |
Average % |
95 |
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| Range % |
93-100 |
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回收率
| The recovery of rat Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-102 |
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| EDTA plasma (n=4) |
94 |
85-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.121 |
0.116 |
0.119 |
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| 2000 |
0.223 |
0.230 |
0.227 |
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| 1000 |
0.468 |
0.475 |
0.472 |
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| 500 |
0.774 |
0.785 |
0.78 |
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| 250 |
1.225 |
1.213 |
1.219 |
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| 125 |
1.689 |
1.674 |
1.682 |
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| 62.5 |
2.162 |
2.179 |
2.171 |
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| 0 |
2.608 |
2.594 |
2.601 |
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货期
3-5 working days