产品描述
This Rat PDGF-CC ELISA Kit was designed for the quantitative measurement of Rat PDGF-CC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
缩写
PDGF-CC
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 85-95 | | |
| 1:2 | Average % | 103 | | |
| Range % | 99-112 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:8 | Average % | 90 | | |
| Range % | 85-94 | | |
回收率
| The recovery of rat PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 87 | 82-93 | | |
| EDTA plasma (n=4) | 95 | 90-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.143 | 2.003 | 2.073 | 1.893 | | | | 500 | 1.487 | 1.405 | 1.446 | 1.266 | | | | 250 | 0.899 | 0.927 | 0.913 | 0.733 | | | | 125 | 0.603 | 0.569 | 0.586 | 0.406 | | | | 62.5 | 0.391 | 0.412 | 0.402 | 0.222 | | | | 31.2 | 0.270 | 0.284 | 0.277 | 0.097 | | | | 15.6 | 0.240 | 0.231 | 0.236 | 0.056 | | | | 0 | 0.183 | 0.176 | 0.180 | | | |
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货期
3-5 working days