产品描述

This Human PDIA4 ELISA Kit was designed for the quantitative measurement of Human PDIA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

Calcium binding protein intestinal related ELISA Kit; Endoplasmic reticulum resident protein 70 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; EPR70 ELISA Kit; EPR72 ELISA Kit; ER protein 70 ELISA Kit; ER protein 72 ELISA Kit; ERP 70 ELISA Kit; ERp 72 ELISA Kit; ERp-72 ELISA Kit; ERp70 ELISA Kit; ERp72 ELISA Kit; PDIA 4 ELISA Kit; Pdia4 ELISA Kit; PDIA4_HUMAN ELISA Kit; PDIR ELISA Kit; Protein disulfide isomerase A4 ELISA Kit; Protein disulfide isomerase associated 4 ELISA Kit; Protein disulfide isomerase family A member 4 ELISA Kit; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ELISA Kit; Protein disulfide isomerase related protein ELISA Kit; Protein disulfide-isomerase A4 ELISA Kit; Protein ERp 72 ELISA Kit; Protein ERp72 ELISA Kit

缩写

PDIA4

Uniprot No

P13667

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		89-97
1:2		Average %		100
		Range %		96-104
1:4		Average %		90
		Range %		88-93
1:8		Average %		91
		Range %		86-94

回收率

The recovery of human PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		87-94
EDTA plasma (n=4)		104		99-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 1.960 1.977 1.969 1.817 750 1.284 1.300 1.292 1.140 375 0.725 0.713 0.719 0.567 187.5 0.412 0.406 0.409 0.257 94 0.287 0.277 0.282 0.130 47 0.220 0.215 0.218 0.066 23.5 0.184 0.178 0.181 0.029 0 0.150 0.154 0.152

货期

3-5 working days