产品描述
This Rat TPO Ab ELISA Kit was designed for the quantitative measurement of Rat TPO Ab protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 5 IU/mL-80 IU/mL and the sensitivity is 5 IU/mL.
缩写
TPO Ab
灵敏度
5 IU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
86-101 |
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| 1:2 |
Average % |
88 |
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| Range % |
83-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-100 |
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回收率
| The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-99 |
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| EDTA plasma (n=4) |
95 |
90-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| IU/ml |
OD1 |
OD2 |
Average |
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| 80 |
0.171 |
0.169 |
0.17 |
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| 40 |
0.285 |
0.276 |
0.281 |
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| 20 |
0.495 |
0.505 |
0.5 |
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| 10 |
1.037 |
1.014 |
1.026 |
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| 5 |
2.032 |
2.066 |
2.049 |
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货期
3-5 working days