产品描述

This Human TNFSF13 ELISA Kit was designed for the quantitative measurement of Human TNFSF13 protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

别名

A proliferation inducing ligand ELISA Kit; A proliferation-inducing ligand ELISA Kit; APRIL ELISA Kit; CD256 ELISA Kit; TALL-2 ELISA Kit; TALL2 ELISA Kit; TNF related death ligand ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 2 ELISA Kit; TNF-related death ligand 1 ELISA Kit; TNF13_HUMAN ELISA Kit; TNFSF13 ELISA Kit; TRDL-1 ELISA Kit; TRDL1 ELISA Kit; Tumor necrosis factor (ligand) superfamily; member 13 ELISA Kit; Tumor necrosis factor ligand superfamily member 13 ELISA Kit; Tumor necrosis factor like protein ZTNF2 ELISA Kit; Tumor necrosis factor related death ligand 1 ELISA Kit; UNQ383/PRO715 ELISA Kit; ZTNF2 ELISA Kit

缩写

TNFSF13

Uniprot No

O75888

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human APRIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		92
		Range %		88-97
1:4		Average %		86
		Range %		82-90
1:8		Average %		98
		Range %		94-102

回收率

The recovery of human APRIL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		100-108
EDTA plasma (n=4)		97		92-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   50 2.285 2.352 2.319 2.179   25 1.605 1.565 1.585 1.445   12.5 1.053 1.104 1.079 0.939   6.25 0.656 0.679 0.668 0.528   3.12 0.411 0.396 0.404 0.264   1.56 0.298 0.318 0.308 0.168   0.78 0.212 0.209 0.211 0.071   0 0.143 0.137 0.140

货期

3-5 working days