产品描述

This Human PLA2G5 ELISA Kit was designed for the quantitative measurement of Human PLA2G5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.04 ng/mL.

别名

Ca2+ dependent phospholipase A2 ELISA Kit; Calcium dependent phospholipase A2 ELISA Kit; Calcium-dependent phospholipase A2 ELISA Kit; DKFZp686C2294 ELISA Kit; FRFB ELISA Kit; Group V phospholipase A2 ELISA Kit; GV PLA2 ELISA Kit; gVPLA2 ELISA Kit; hVPLA(2) ELISA Kit; MGC46205 ELISA Kit; OTTHUMP00000044655 ELISA Kit; PA2G5_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 5 ELISA Kit; Phospholipase A2 group V ELISA Kit; PLA2 10 ELISA Kit; PLA2 G5 ELISA Kit; PLA2-10 ELISA Kit; Pla2g5 ELISA Kit; sPLA2 Type V ELISA Kit

缩写

PLA2G5

Uniprot No

P39877

灵敏度

0.04 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		89
		Range %		84-93
1:10		Average %		98
		Range %		84-102
1:20		Average %		104
		Range %		100-110
1:40		Average %		95
		Range %		90-100

回收率

The recovery of human PLA2G5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		84		80-88
EDTA plasma (n=4)		96		91-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 12 2.207 2.150 2.179 2.023 6 1.768 1.801 1.785 1.629 3 1.202 1.184 1.193 1.037 1.5 0.683 0.634 0.659 0.503 0.75 0.403 0.428 0.416 0.260 0.375 0.321 0.301 0.311 0.155 0.18 0.245 0.227 0.236 0.080 0 0.159 0.152 0.156

货期

3-5 working days